FIG 5.

Number of DC-SIGN⁺ MΦ is positively correlated with MΦ-mediated cis and trans infection efficiency. (A) SN MΦ-mediated trans infection efficiency and percentage of DC-SIGN-positive MΦ were assessed for association by linear regression (P = 0.03, F = 7.18, R² = 0.47) (n = 10). (B and C) Anti-DC-SIGN- and isotype control-treated SN MΦ were loaded with HIV-1 BaL (MOI, 10⁻¹) and cultured alone, or loaded at an MOI of 10⁻³ and cultured with activated autologous CD4⁺ T cells, to assess cis (B) and trans (C) infection (n = 5). (D) SN MΦ were treated with a MAb against CD209 (DC-SIGN) or an isotype control and then assessed by flow cytometry for DC-SIGN detectability (n = 2). *, P ≤ 0.05. Histograms represent the means ± SE.