TABLE 2.
Mutant | Primers used to introduce mutationsa |
---|---|
TFV/AAA | 5′-CAGCGAGTCGCGATCGGCGGCGGCCATAGGAATTTTCCCTTCGC-3′ |
5′-GCCGCCGCCGATCGCGACTCGCTG-3′ | |
DR/AA | 5′-CAGCGAGTCGGCGGCCACGAAGGTCATAGGAATTTTCCCTTCGC-3′ |
5′-ACCTTCGTGGCCGCCGACTCGCTG-3′ | |
DS/AA | 5′-ATTGGCTCGCAGGGCGGCGCGATCCACGAAGGTCATAGGAATTT-3′ |
5′-GTGGATCGCGCCGCCCTGCGAGCCAAT-3′ |
Underlining indicates nucleotide substitutions introduced into the pUL38-coding sequence.