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. 2018 Jun 13;92(13):e00229-18. doi: 10.1128/JVI.00229-18

FIG 7.

FIG 7

SUN1 inhibition of HIV-1 and HIV-2 infections is dependent on CA-CypA interaction. (A) Sequences of the cyclophilin A binding loop (CBL) of the CA proteins of HIV-1 and SIVtan. (B) HEK293A cells were treated with CsA or DMSO and infected with the same amounts of the indicated VSV_G-pseudotyped virion particles. At 48 h postinfection, luciferase activity was measured. CBL-chimera, the CBL of NL4-3luc was replaced with the counterpart of SIVtan. Fold inhibition (FI) was calculated as the luciferase activity in the DMSO-treated cells divided by that in the CsA-treated cells and is indicated above the bars. (C) HEK293A cells transduced with an empty lentivector (EV) or a lentivector expressing SUN1-Flag were infected with the same amounts of the indicated VSV_G-pseudotyped virion particles. Luciferase activity was measured at 48 h postinfection. Fold inhibition (FI) was calculated as the luciferase activity in the control cells divided by that in the SUN1-expressing cells and is indicated above the bars. (D) HEK293A cells transduced with an empty lentivector or a lentivector expressing SUN1-Flag were treated with increasing concentrations of CsA and infected with VSV_G-pseudotyped NL4-3luc. At 48 h postinfection, luciferase activity was measured. Fold inhibition was calculated as the luciferase activity in the control cells divided by that in the SUN1-expressing cells. (E) HEK293A cells were treated with DMSO or CsA at the indicated concentrations and then infected with the indicated VSV_G-pseudotyped viruses. At 48 h postinfection, luciferase activity was measured. (F) Sequences of the CBL of HIV-1 and HIV-2. (G) HEK293A cells transduced with an empty lentivector (EV) or a lentivector expressing SUN1-Flag were challenged with the indicated VSV_G-pseudotyped virion particles. Luciferase activity was measured at 48 h postinfection. Fold inhibition (FI) was calculated as described for panel C and is indicated above the bars. (H) HEK293A cells transduced with an empty lentivector (EV) or a lentivector expressing TRIMCyp-Flag were infected with the indicated VSV_G-pseudotyped virion particles and treated with CsA or DMSO. At 48 h postinfection, luciferase activity was measured. Fold inhibition (FI) was calculated as the luciferase activity in the control cells divided by that in the TRIMCyp-expressing cells and is indicated above the bars. Data presented are means and SD for three independent experiments.