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Schematic diagram (A) and SDS-PAGE analysis (B) of various sORF2-C truncated fragments expressed using a prokaryotic system. Recombinant plasmid containing the gene sequence encoding the C-terminal 268 amino acids of genotype 4 swine HEV was used as the template for construction of eight truncated ORF2 fragments, as described in the text. Overlapping amino acid regions of the various truncated fragments are shown in the schematic diagram in panel A.
