TABLE 2.
Synthetic oligonucleotide primers used in the studya
| Oligonucleotide | Sequenceb |
|---|---|
| sORF2-393F | CGCGGATCCGCAGTTATTTTACTCC |
| sORF2-407F | CGCGGATCCGGAGCCGACAGTAAAA |
| sORF2-411F | CGCGGATCCGCTCTACACTTCAGTC |
| sORF2-458F | CGCGGATCCGTCTCGTCCTTTCTCT |
| sORF2-464F | CGCGGATCCGTCCTTCGTGCCAAT |
| sORF2-527F | CGCGGATCCGACCATCCAGCAGTAT |
| sORF2-526R | CCCAAGCTTTCAAGTGAGCGGGCGACC |
| sORF2-660R | CCCAAGCTTTCAATACTCCCGGGT |
| sORF2-624R | CCCAAGCTTTCAATCAAAAGTATGGGC |
| sORF2-628R | CCCAAGCTTTCAAGGGCAGAAATCATC |
| sp239-F | CCCCCATATGATAGCGCTAACTTTGT |
| sp239-R | TTCCAAGCTTTCACGCAGTATGGGGGGCG |
| Sar-55-239-F | CCCCCATATGATAGCGCTTACCCTGT |
| Sar-55-239-R | TTCCAAGCTTTCACACAGAGTGGGGGGCT |
| Kernow C1-239-F | CCCCCATATGATCGCCCTGACACTGT |
| Kernow C1-239-R | TTCCAAGCTTTCAGGCCGAGTGTGGGGCT |
| rHEV-239-F | CCCCCATATGATAGCCCTGACGCTGT |
| rHEV-239-R | TTCCGGATCCTCAGACAGAATGTGGAGCG |
a
The oligonucleotide primers were used for cloning and expression of different truncated fragments of genotype 4 swine HEV capsid protein and target regions of amino acids 368 to 606 of capsid proteins from various swine, human, and rabbit HEV strains.
b
Underlining indicates BamHI, NdeI, or HindIII sequences.