TABLE 3.
Sequences of the synthetic peptides used to map the minimal MAb binding motifs
| Short peptide | No. of amino acids | Purity (%) | Sequence (N terminus to C terminus)a | Interact with relative MAb (OD450) |
|---|---|---|---|---|
| Pep2G9-1 | 6 | 96.5 | GEPTVK | 1.09 |
| Pep2G9-2 | 6 | 95.7 | NGEPTV | 1.11 |
| Pep2G9-3 | 6 | 98.0 | PTVKLY | 0.25 |
| Pep2G9-4b | 6 | 95.6 | ANGEPT | 0.21 |
| Pep2C7-1 | 6 | 95.6 | PSRPFS | 1.00 |
| Pep2C7-2 | 6 | 95.2 | SRPFSV | 0.33 |
| Pep2C7-3 | 6 | 98.8 | APSRPF | 1.24 |
| Pep1E4-1 | 6 | 95.6 | FDDFCP | 1.01 |
| Pep1E4-2 | 6 | 98.6 | TFDDFC | 0.42 |
| Pep1E4-3 | 6 | 96.5 | HTFDDF | 0.21 |
a
Bold letters indicate key amino acids determined by truncated sORF2-C protein.
b
Pep2G9-4 was used to identify whether V470 is a common amino acid in the epitopes shared by 2G9 and 1B5.