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. 2018 Jun 13;92(13):e01891-17. doi: 10.1128/JVI.01891-17

FIG 13.

FIG 13

Ex vivo peptide/tetramer bead-based enrichment of L-HDAg104-112-specific CD8+ T cells in patients with resolved versus chronic HDV infections. (A) L-HDAg104-112 peptide/HLA-B*27:05 tetramer bead-based ex vivo enrichment of HDV-specific CD8+ T cells was performed for five patients with chronic HBV/HDV coinfections, as well as one patient with chronic HBV infection and resolved HDV infection (32). Using this extremely sensitive method, we were able to detect HDV-specific CD8+ T cells from a patient with chronic HBV infection and resolved HDV infection. In contrast, we were not able to enrich HDV-specific CD8+ T cells from the five patients with chronic HBV-HDV coinfections. The autologous sequences are also displayed (ND, viral sequencing not done due to low viral load). (B) Ex vivo-enriched HDV-specific CD8+ T cells from the patient with chronic HBV infection and resolved HDV infection were further analyzed for expression of PD1 and CD127. Naive CD8+ T cells were excluded by costaining for CCR7 and CD45RA.