TABLE 5.
Primers used in L-HDAg amplification for direct and deep sequencing
| Primer | Sequence | Location (nt) |
|---|---|---|
| 771R | 5′-CGGTCCCCTCGGAATGTTG-3′ | 753–771 |
| 339R | 5′-GCTGAAGGGGTCCTCTGGAGGTG-3′ | 319–341 |
| 1674R | 5′-AGAAAAGAGTAAGAGYACTGAGG-3′ | 1652–1674 |
| 912F | 5′-GAGATGCCATGCCGACCCGAAGAG-3′ | 912–936 |
| 891F | 5′-AGGTCGGACCGCGAGGAGGT-3′ | 891–910 |
| M13 HDV 956a | 5′-GTTGTAAAACGACGGCCAGTTCACTGGGGTCGACAACTCTG-3′ | |
| M13 HDV 1360a | 5′-CACAGGAAACAGCTATGACCGTAGACTCCGGACCTAGGAAGA-3′ | |
| OligoAMIDM13fwb | 5′-CGTATCGCCTCCCTCGCGCCATCAGMIDGTTGTAAAACGACGGCCAGT-3′ | |
| OligoBMIDM13rvb | 5′-CTATGCGCCTTGCCAGCCCGCTCAGMIDCACAGGAAACAGCTATGACC-3′ |
a
M13 HDV 956 and M13 HDV 1360 primers consist of M13 fagus universal tail at the 5´ ends followed by HDV-specific sequences (underlined).
b
Specific oligonucleotides for UDPS are indicated in bold followed by multiplex identifiers (MID) and the M13 fagus universal tails.