FIG 5.

Systemic production of IFN-α and IL-6 is dependent on IPS-1 and MyD88 during SFTSV infection in vivo. (A) IFN-α and IL-6 production levels in sera from SFTSV-infected mice at the indicated time points. (B) Survival rates of WT, IPS-1^−/−, MyD88^−/−, IPS-1^−/− MyD88^−/−, and IFNAR1^−/− mice after SFTSV challenge. (C) SFTSV titers in peripheral blood of infected mice at 2 dpi. (D) Immunohistochemical examination of SFTSV N in the spleens of WT, IFNAR1^−/−, and IPS-1^−/− MyD88^−/− mice at 2 dpi. Spleen sections were costained for SFTSV N (green) and DAPI (blue) and analyzed by confocal microscopy. Scale bars, 250 μm. The results are representative of 3 mice. (A and C) The data are representative of the results of two independent experiments and are plotted as means ± SD. (A) Unpaired Student t tests were used to determine significant differences in IFN-α and IL-6 production between samples at 24 hpi and 48 hpi, respectively. *, P < 0.05; **, P < 0.01; #, not significant.