TABLE 3.
Prediction of the putative polyprotein pp1a/pp1ab cleavage sites of novel MERS-related CoVs based on comparison with MERS-CoV strain EMC/2012
| Nspa | BtCoV/Ii/GD/2013-845 |
BtCoV/Ii/GD/2014-422 |
Putative functional domain(s)b | ||
|---|---|---|---|---|---|
| First-last amino acid residues | Protein size (aa) | First-last amino acid residues | Protein size (aa) | ||
| 1 | Met1-Gly196 | 196 | Met1-Gly196 | 196 | IFN antagonist |
| 2 | Asp197-Gly856 | 660 | Asp197-Gly856 | 660 | |
| 3 | Ala857-Gly2754 | 1,898 | Ala857-Gly2768 | 1,912 | ADRP, PL2pro |
| 4 | Ala2755-Gln3261 | 507 | Ala2769-Gln3275 | 507 | TM-2 |
| 5 | Ser3262-Gln3567 | 306 | Ser3276-Gln3581 | 306 | 3CLpro |
| 6 | Ser3568-Gln3859 | 292 | Ser3582-Gln3873 | 292 | TM-3 |
| 7 | Ser3860-Gln3942 | 83 | Ser3874-Gln3956 | 83 | |
| 8 | Ala3943-Gln4141 | 199 | Ala3957-Gln4155 | 199 | Primase |
| 9 | Asn4142-Gln4251 | 110 | Asn4156-Gln4265 | 110 | RNA-binding protein |
| 10 | Ala4252-Gln4391 | 140 | Ala4266-Gln4405 | 140 | ZBD, cofactor of OMT |
| 11 | Ser4392-Ile4395 | 14 | Ser4406-Ile4419 | 14 | Short peptide |
| 12 | Ser4392-Gln5325 | 934 | Ser4406-Gln5339 | 934 | RdRp |
| 13 | Ala5326-Gln5923 | 598 | Ala5340-Gln5937 | 598 | Hel, NTPase |
| 14 | Ser5924-Gln6447 | 524 | Ser5938-Gln6461 | 524 | ExoN, NMT |
| 15 | Gly6448-Gln6970 | 343 | Gly6462-Gln6804 | 343 | NendoU |
| 16 | Ala6969-Arg7093 | 303 | Ala6805-Cys7107 | 303 | OMT |
a
Nsp, nonstructural protein.
b
ADRP, ADP-ribose 1″-phosphatase; PL2pro, papain-like protease 2; TM, transmembrane domain; 3CLpro, coronavirus Nsp5 protease; ZBD, zinc-binding domain; Hel, helicase; NTPase, nucleoside triphosphatase; ExoN, exoribonuclease; NMT, N7 methyltransferase; NendoU, endoribonyuclease; OMT, 2′O-methyltransferase.