FIG 3.

Impact of the molecular strategy for LucR reporter element insertion on Env conformation. Cell surface staining of primary CD4⁺ T cells either mock infected or infected with IMCs expressing different Env strains (CH58, CH40, CH77, CH505, YU2, SF162, and BaL) and expressing or not the LucR reporter gene with A32 (A to D) or HIV⁺ sera (E to H). (A to C and E to G) Histograms depicting representative A32 or HIV⁺ serum staining (A and E) and the MFI in the infected (p24⁺) population (B, C, F, and G) obtained in at least 5 independent experiments. (D and H) Spearman rank correlations between the levels of cell surface CD4 (detected with the anti-CD4 Ab OKT4) and Env staining performed with A32 or HIV⁺ sera using different IMC constructs. The data are shown as means and SEM. Statistical significance was tested using an unpaired t test (B, C, F, and G) or a Spearman correlation test (D and H) (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001).