FIG 1.

Schematic representation of an 11-plasmid system for RVA reverse genetics. The 11 RVA rescue T7 plasmids contain the full-length segment cDNA of an individual gene of SA11-L2 virus, flanked by the T7 RNA promoter (PT7) and the HDV ribozyme (Rib). BHK/T7-9 cells were transfected with the 11 RVA plasmids with/without increased amounts of the two plasmids carrying the NSP2 and NSP5 genes. Recombinant RVAs were rescued from the cultures of the transfected BHK/T7-9 cells by amplification (A) or plaque formation (B) on CV-1 cells.