FIG 2.

Electron microscopy of cells with recombinant RVAs. BHK/T7-9 cells were transfected with 11 RVA plasmids with 0.75 μg of each plasmid (1-fold) (A) or with 11 RVA plasmids with increased amounts of the plasmids carrying the NSP2 and NSP5 genes, from 0.75 to 2.25 μg (3-fold) (B). (C) BHK/T7-9 cells were infected with the native SA11-L2 virus at an MOI of 3. At 24 h after transfection or at 8 h after infection, cells were fixed and stained and then observed under a transmission electron microscope. Bars represent 500 nm.