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. 2018 Jun 13;92(13):e00588-18. doi: 10.1128/JVI.00588-18

FIG 4.

FIG 4

Genetic stability of the recombinant rSA11-Nluc virus. rSA11-Nluc was consecutively passaged 10 times in MA104 cells. (A) PAGE of viral genomic dsRNAs extracted from passage 1 (P1), 3 (P3), 5 (P5), and 10 (P10) stocks of rSA11-Nluc. Lane 1, dsRNAs from rSA11-L2; lanes 2, 3, 4, and 5, dsRNAs from P1 (lane 2), P3 (lane 3), P5 (lane 4), and P10 (lane 5) stocks of rSA11-Nluc. The numbers on the left indicate the order of the genomic dsRNA segments of rSA11-L2. (B) Nluc activities of P1, P3, P5, and P10 stocks of rSA11-Nluc. MA104 cells were infected with rSA11-L2 or rSA11-Nluc at an MOI of 0.01 and incubated for 12 h, and then Nluc activities in the cultures were determined by luminometry. The data shown are the mean Nluc activities and SDs for three independent cell cultures.