Infectivity of the recombinant rSA11-EGFP and rSA11-mCherry viruses. (A) Multiple-step growth curves for rSA11-L2, rSA11-EGFP, and rSA11-mCherry. MA104 cells were infected with rSA11-L2, rSA11-EGFP, or rSA11-mCherry at an MOI of 0.01 and then incubated for various numbers of hours. Virus titers in the cultures were determined by plaque assay. The data shown are the mean viral titers and SDs for three independent cell cultures. *, P < 0.05 (as determined by t test). (B) Plaque formation by rSA11-L2, rSA11-EGFP, and rSA11-mCherry. rSA11-L2, rSA11-EGFP, or rSA11-mCherry was directly plated onto CV-1 cells for plaque formation. The experiment was repeated three times with similar results, and representative results are shown.