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. 2018 Jun 13;92(13):e00588-18. doi: 10.1128/JVI.00588-18

FIG 7.

FIG 7

Genetic stability of the recombinant rSA11-EGFP and rSA11-mCherry viruses. rSA11-EGFP and rSA11-mCherry were consecutively passaged 10 times in MA104 cells. (A) PAGE of viral genomic dsRNAs extracted from P1, P3, P5, and P10 stocks of rSA11-EGFP and rSA11-mCherry. Lanes 1 and 6, dsRNAs from rSA11-L2; lanes 2, 3, 4, and 5, dsRNAs from P1 (lane 2), P3 (lane 3), P5 (lane 4), and P10 (lane 5) stocks of rSA11-EGFP; and lanes 7, 8, 9, and 10, dsRNAs from P1 (lane 7), P3 (lane 8), P5 (lane 9), and P10 (lane 10) stocks of rSA11-mCherry. The numbers on the left indicate the order of the genomic dsRNA segments of rSA11-L2. (B) RT-PCR analysis of engineered NSP1 segments of rSA11-EGFP and rSA11-mCherry. The NSP1 genes were amplified by RT-PCR using viral genomic dsRNAs and specific primers for the NSP1, EGFP, and/or mCherry nucleotide sequences for successive virus passage P1, P3, P5, and P10 stocks of rSA11-EGFP and rSA11-mCherry, followed by separation in a 2% agarose gel. DNA markers are 1,500, 1,000, 900, 800, 700, 600, and 500 bp.