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. Author manuscript; available in PMC: 2019 May 22.
Published in final edited form as: Biochemistry. 2018 May 10;57(20):2958–2970. doi: 10.1021/acs.biochem.8b00226

Figure 6.

Figure 6

(A) The 5-track G4 sequence of the NEIL3 was replaced with the 5-track G4 sequences of c-MYC, VEGF and hTERT. DNA templates were annealed to a radiolabeled 15-mer primer P. Expected arrest site at the G4 site is marked as Q while the fully synthesized duplex DNA for c-MYC, VEGF and hTERT are designated as FC, Fv and FT, respectively. (B) Autoradiograph of the DNA polymerase stop assay. Primer annealed-template DNAs were incubated at 37 °C for 30 min with (+) or without (−) the Klenow Fragment enzyme E. All even-numbered lanes were reacted in buffered physiological salts while the odd-numbered lanes were assayed in LiCl buffer.