Skip to main content
. Author manuscript; available in PMC: 2018 Jun 15.
Published in final edited form as: Nitric Oxide. 2017 Sep 4;70:59–67. doi: 10.1016/j.niox.2017.09.001

Fig. 6. CR-geNOp, a ratiometric NO probe based on two FPs.

Fig. 6

(A) Schematic structures of CR-geNOp consisting of the cyan FP (CFP), a NO-sensitive GAF domain, a rigid linker (EAAAK)n and the red FP (tagRFP) in the presence and absence of NO, which quenches CFP fluorescence only (B) Left panel: fluorescence intensity CFP (cyan curve) and RFP (red curve) over time in cells expressing CR-geNOp. Cells were treated with 10 μM NOC-7 as indicated. Right panel: respective ratio signal (red/cyan; F584/F480) of CR-geNOp (n = 98/5). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)