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. Author manuscript; available in PMC: 2019 Jul 1.
Published in final edited form as: Wiley Interdiscip Rev RNA. 2018 May 3;9(4):e1477. doi: 10.1002/wrna.1477

Figure 5.

Figure 5

Traditional and emerging screens to identify small molecule miRNA inhibitors. (a) A standard luciferase-based reporter system used to identify small molecule miRNA inhibitors. Increase in luciferase signal indicative of translation is assumed to be caused by small molecule binding to miRNA or one if its precursors, thereby reducing binding of the mature miRNA to its target sequence. Adapted from Mahato et al.97 (b) A click-chemistry-based assay to identify small molecule inhibitors of Dicer-mediated processing. Adapted from Garner et al.108 Abbreviations: RISC, RNA-induced silencing complex.