Table 1.

EC50 and LC50 values (168 hpf, with the exception of PTU 120 hpf), as well as the p-values indicating significant differences between LC50 and EC50 values, after exposure experiments (six concentrations per compound, 40 embryos per condition) at UA (effects on posterior chamber inflation).

Compound	EC50 (mg/L)	LC50 (mg/L)	p-value	Observed impaired posterior chamber inflationa	Concentration at which effect on posterior chamber surface area (120 hpf) occursb
PTU	259	555.9	<0.0001	X	EC50
IOP	2.791	3.471	0.0448	X	No data
PFOA	108.5	362.5	0.0150	X	EC10, EC30, EC50
BPA	4.7	8	0.0427	X	EC10, EC30, EC50
BP2	10.55	15.48	<0.0001	X	No data
TBP	0..42	0.84	<0.0001	X	EC10, EC30, EC50
SA	82.6	137.3	<0.0001	X	EC10, EC30, EC50
PFOS (Hagenaars et al., 2016)	2.12	8.35	<0.0001	X	No data
ANS	112.2	124.7	0.0606	–	EC10, EC30, EC50
TCBPA	0.43	0.41	0.0687	–	No data
NaPER	3435	2952.7	<0.0001	–	No effect
MMI	772.0	894.8	0.1395	–	EC10, EC30, EC50 *No effect at 168 hpf
PFBS	830.3	921.9	0.3171	–	No effect
MBT (Stinckens et al., 2016)	3.2	4.2	0.1529	–	No effect

^aObserved effects on binary scoring of the absence of posterior chamber inflation (X = effect = significant difference between EC50 and LC50 values, UA) are given. Based on the exposure experiments at UA, EC10, EC30 and EC50 values were determined at 120 hpf and used to perform exposure experiments at UCR (three concentrations per compound, at least 40 embryos per condition, effects on posterior surface area).

^bObserved effects on posterior surface area (effect concentration, UCR, see section 3.2.2) are given.