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. 2018 Jun 15;10:78. doi: 10.1186/s13148-018-0512-1

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Expression of immune checkpoints and PD-L1 genes and methylation/demethylation genes in breast tumor and normal tissues. RNA isolated from breast tumor and normal tissues from eight patients were reverse transcribed to cDNA. Quantitative RT-PCR was performed to assess the expression level of PD-1, PD-L1, CTLA-4, TIGIT, TIM-3, and LAG-3 (a); TET1, TET2, TET3, DNMT3a, and DNMT3b (c) from both NT and TT. The relative expression of each gene was normalized to β-actin. Absolute expression of each gene was calculated by normalizing each gene to TIGIT expression (b)