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. 2018 Mar 15;29(6):736–750. doi: 10.1091/mbc.E17-08-0518

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FIGURE 7: — Localization of exocyst subunits in membrane anchored Exo70p or Sec3p strains. (A) A GFP tag was added to Exo70p or Sec3p in WT and TM anchored EXO70 or SEC3 strains. Yeast cells were grown to early log phase in selection medium at 25°C and harvested, and the live cells were directly examined by fluorescence microscopy. Bars, 5 µm. (B) A GFP tag was added to Sec5p, Sec8p, or Exo84p in WT and TM-anchored EXO70 or SEC3 strains. Yeast cells were grown to early log phase in selection medium at 25°C and harvested, and the live cells were directly examined by fluorescence microscopy. Bars, 5 µm.