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. 2018 Mar 15;29(6):722–735. doi: 10.1091/mbc.E17-06-0412

FIGURE 1:

FIGURE 1:

SAS-5 weakly localizes to the mitotic spindle. (A, B) DIC (top panels) and GFP fluorescence (bottom panels) images of an embryo expressing GFP-SAS-5, at metaphase of the one-cell stage (A) and at metaphase of the AB blastomere in the two-cell stage (B, left cell). See Supplemental Movie 1. (C–F) Immunofluorescence of wild-type (N2) embryos from worms grown without RNAi (C, D) or upon sas-5(RNAi) (E, F). Top panels show SAS-5 (green), α-tubulin (magenta), and DNA (blue); bottom panels show SAS-5 only. Images are maximum-intensity z-projections. White arrows indicate SAS-5 spindle staining; white arrowheads, centrioles; cyan arrows, membrane/cortical staining; and red arrow, nonspecific P-granule staining. SAS-5 localizes to centrioles, as well as to the mitotic spindle, most notably proximal to kinetochores, and at the cell cortex, together suggesting that SAS-5 exhibits affinity for microtubule plus ends. Scale bars, 10 μm. All embryos are oriented with the anterior on the left and the posterior on the right.