TABLE 1:

Microtubule-binding properties of SAS-5 constructs and variants.

Constructa	Microtubule colocalization in COS-7 cells	Microtubule-pelleting assays	DrCPAPG-box interaction by ITC
1–404	+	NDb	ND
1/2-265 (SAS-52–265)	+	+++	ND
70–265	+	ND	ND
90–265	–	ND	ND
1–180	–	ND	ND
SAS-52–265 L141E	–	+	ND
SAS-52–265 I247E	–	+	ND
SAS-52–265 L141/I247E	–	–	ND
SAS-52–265 ∆72–80	ND	++	ND
SAS-52–265 ∆81–90	ND	+	ND
SAS-52–265 ∆91–100	ND	++	ND
SAS-52–265 ∆101–110	ND	+++	ND
SAS-52–265 K65E/K66E/K67E	ND	–	ND
SAS-52–265 R86E/K88E/K94E	ND	–	ND
2–122 (SAS-5N)	ND	+/−	+++
SAS-5N ∆72–80	ND	ND	++
SAS-5N ∆81–90	ND	ND	+++
SAS-5N ∆91–100	ND	ND	+++
SAS-5N ∆101–110	ND	ND	+++
SAS-5N K65E/K66E/K67E	ND	ND	++
SAS-5N R86E/K88E/K94E	ND	ND	+

^aDenotes SAS-5 amino acid residue ranges, construct acronyms, amino acid substitutions and/or deletions.

^bND, not determined.