| α-Centromere derived from human CREST patient serum (Antibodies, Inc., 15-234-0001) | 1:100 |
| Custom rabbit α-CENP-A | 2 µg/ml |
| α-Flag M2 (Sigma, F3165) | 2 µg/ml for CENP-C-mRuby2-3xFlag |
| 5 µg/ml for CENP-N-mRuby2-3xFlag truncations | |
| Llama nanobody α-GFP directly conjugated with Alexa Fluor 488 | 1 µg/ml for salt-wash experiments |
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