Fig. 6.

Knockdown of RTN1-C rescues neural apoptosis induced by ROP18. (A) WT or RTN1-C KO Neuro2a cells were infected with ∆ku80∆hxgprt RH or ∆rop18 RH parasites at a multiplicity of infection of ∼3 for 24 h. Then, cell apoptosis was detected by flow cytometry after annexin V-phycoerythrin (PE)/7-aminoactinomycin D (7-AAD) staining. The plots are obtained from a representative measurement. (B) Quantitative data of A are expressed as the mean ± SD based on three different assays (n = 3). **P < 0.01; ***P < 0.001.