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Orthogonal binding assays confirm the activity of 12CA5-binding MIMs. (A) LC-MS analysis of representative biotinylated 12CA5-binding MIM 1. (B) BLI assay, showing association of exogenous 12CA5 to immobilized MIM 1. (C) FP competition binding assay, showing the displacement of fluorescent HA epitope by unlabeled HA epitope (red) and MIM 1 (black).
