Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 May 21;115(23):E5289–E5297. doi: 10.1073/pnas.1805257115

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Published under the PNAS license.

PMC Copyright notice

Fig. 3. — Antagonistic effects of CRTC3 on PRDM16-mediated brown preadipocyte differentiation. (A, Left) Oil Red O staining of cells from the brown stromal vascular fraction (bSVFs) of BKO versus WT (CRTC3 fl/fl) littermates following exposure to differentiation mixture for 7 d. (A, Right) Oil Red O staining of bSVFs from CRTC3 Tg and WT littermates following exposure to differentiation mixture. (B and C) Relative protein (B) and mRNA (C) amounts for thermogenic and myogenic genes in differentiated bSVFs from BKO and WT littermates. (D) Oil Red O and BODIPY staining of C2C12 cells stably expressing PRDM16 after exposure to differentiation mixture for 7 d. Effect of lentiviral CRTC3 overexpression on differentiation of C2C12-PRDM16 cells relative to GFP control shown. (E) Effect of modest CRTC3 overexpression on protein (Left) and mRNA (Right) amounts for thermogenic and myogenic genes in differentiated C2C12-PRDM16 cells. Data in C and E represent means ± SD (*P < 0.05; **P < 0.01).