Table 1.

Summary table of sequencing reads and annotated read statistics

			Calibrators			RNA category, %
Biofluid		Total	Set 1	Set 2	Sample	miRNA	rRNA	tRNA	scRNA	Other cRNA	mRNA	non-hg19	Technical
Serum, n = 96	Average	4,568,021	12,090	18,909	2,984,332	53.4	8.5	6.6	9.7	1.6	5.2	14.8	1,552,689
	Median	2,190,478	6,384	10,202	1,473,132	54.5	7.8	5.6	7.9	1.6	4.6	12.3	602,986
	Min	91,780	214	723	51,795	17.7	1.0	0.3	2.1	0.7	0.8	2.5	25,798
	Max	31,993,329	124,488	193,438	23,141,839	81.6	19.8	20.2	39.1	2.4	21.8	62.2	12,056,077
Plasma, n = 216	Average	3,242,576	7,784	11,448	2,255,037	77.8	2.8	0.6	3.1	1.2	4.7	9.8	968,308
	Median	2,041,473	5,883	8,524	1,411,639	80.9	2.5	0.4	2.5	0.9	3.7	7.0	429,009
	Min	21,955	47	143	13,300	26.6	0.7	0.2	0.2	0.3	0.8	1.1	2,011
	Max	19,671,378	56,088	81,127	15,970,087	94.4	9.2	6.5	17.5	6.6	29.5	58.7	15,767,594

Listed are median, minimal (min), and maximal (max) mapped reads on a per-sample basis for plasma and serum. The technical read category includes reads mapped against plasmid and genome sequences of the expression system used in production of RNA ligase 1 and 2, which are used for the 5′- and 3′-adapter ligations in cDNA library preparation, as well as marker sequences and reaction side products. Individual sample read reports are detailed in Dataset S1.