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. 2018 Jul 10;29(2):111–127. doi: 10.1089/ars.2017.7097

FIG. 5.

FIG. 5.

Hemin increases intracellular hydrogen peroxide in Hmox1+/+ or Hmox1−/− and decreases Alas1 expression but does not affect levels of heme importers. Scheme of the experiment for the assessment of intracellular heme content (A). Heme cellular content measured with spectrophotometry in MSC Hmox1+/+ or Hmox1−/− stimulated for 2 h with hemin (T2) and then after 2 h in hemin-free medium (T4), N = 3 (B). Expression of FLVCR1 (C), Alas1 (D), Hmox1 (E), and Hmox2 (F) in Hmox1+/+ or Hmox1−/− MSCs or fibroblasts stimulated for 6 h with 50 μmol/L hemin. Levels of H2O2 measured with H2DCFDA after 6 (G), 24 (H), or 48 h (I) in Hmox1+/+ or Hmox1−/− MSCs or fibroblasts stimulated with hemin (50 μmol/L). Data are shown as mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 hemin-treated cells versus control; ##p < 0.01, ####p < 0.0001 MSC versus fibroblasts, $p < 0.05, $$p < 0.01, $$$p < 0.001, $$$$p < 0.0001 Hmox1+/+ versus Hmox1−/−. Two-way ANOVA with Bonferroni post-test, N = 3.