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. 2018 May 7;115(21):E4930–E4939. doi: 10.1073/pnas.1721523115

Fig. 2.

Fig. 2.

Mutants of the NAC troika exhibit strong associations with leaf-senescence phenotypes. (A and B) Relative changes in chlorophyll content (A) and photochemical efficiency (Fv/Fm) (B) in single mutants of the NAC troika (anac017, anac082, and anac090 mutants) compared with wild-type (Col) plants during leaf aging. Values are means ± SD (n > 20) and are normalized to those at 14 d in individual mutants. Colors represent the data measured from the indicated single mutants. (C) Representative photographs of a wild-type plant (Col) and single mutants at 30 d after the emergence of the third or fourth leaves. (Scale bar, 1 cm.) (D) Leaf yellowing in wild-type plants and single mutants at the indicated leaf age (in days). (E) Trypan blue staining of leaves at the indicated leaf age. In each plant, blue-colored patches of cells were developed by Trypan blue staining, indicating areas of dead or dying cells. (F and G) mRNA expression levels of SEN4 (F) and SAG12 (G) in wild-type plants and single mutants at the indicated leaf age. The mRNA expression level of each gene was measured by qRT-PCR and normalized by that of ACT2. Values are means ± SD (n = 2).