Fig. 3.

Sucnr1 is required for succinate-induced type 2 immune responses. Jejunum tissues were collected from Sucnr1^+/− and Sucnr1^−/− mice after consumption of water or water containing 100 mM succinate. (A) The tissues were immunostained with anti-Dclk1 (green) and anti–E-cadherin (red) antibodies and counterstained with DAPI (blue). (Scale bar, 50 μm.) (B) Dclk1-positive cells per mm of villus (n = 6 for each group). (C) Goblet cell staining with Alcian blue/nuclear red. (Scale bar, 50 μm.) (D) Goblet cell number per mm villus (n = 6 for each group). (E) Goblet cell size (n = 6 for each group). All data are biological replicates. **P < 0.01, ***P < 0.001; NS, not significant (Student’s t test). Data are mean ± SEM.