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. 2018 May 3;115(21):5612–5617. doi: 10.1073/pnas.1721110115

Fig. 4.

Fig. 4.

Persistent shade modifies auxin perception and signaling to promote growth. Effects of (A) CS and PS on MSG2/IAA19 and IAA29 expression in Columbia wild type and pif4, (B and C) GUS staining driven by the pAFB2:AFB2-GUS, pTIR1:TIR1-GUS, or pAFB2:AFB2m-GUS transgenes, (D and E) GUS staining driven by the pMIR393a:GUS transgene, and (F and G) fluorescence driven by the pARF6:ARF6-GFP transgene. (In C and E, scale bar = 200 μm. In G, scale bar = 35 μm.) (H) Observed hypocotyl growth (SI Appendix, Table S2) vs. values predicted by a model based on MSG2/IAA19, IAA29, ARF6, AFB2, and TIR1 levels as affected by CS and PS. (I) Goodness-of-fit of the models [as denoted by low Obs-pred/Obs ratios and the χ2 values (numbers on bars)] when considering either CS alone or CS and PS. (J) Enhanced expression of TIR1 by Dexamethasone induction is enough to promote hypocotyl growth. Means ± SE of (A, D, and F) five and (B) seven biological replicates or (J) 15 seedlings, and (C, E, and G) representative images. *P < 0.05; **P < 0.01; ***P < 0.001.