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. 2018 Apr 23;115(21):E4815–E4822. doi: 10.1073/pnas.1802749115

Fig. 6.

Fig. 6.

Displacement of organizer formation can lead to pigment asymmetry in whole embryos. (A) Diagram of experiment in which organizer formation is blocked by βCat antisense MO and subsequently a new organizer displaced by 90° (dark blue) is induced by injecting Siamois mRNA. Gray arrow indicates the direction of gastrulation. (B) Control embryo; note uniform pigmentation of the epidermis (n = 46). (C) Embryo injected with βCat MO showing a ventralized phenotype lacking all axial structures due to loss of Spemann’s organizer (n = 50). (D) Embryo injected with βCat MO and rescued by injection of Siamois mRNA into the vegetal tier of segments 2 and 3 injected embryo at the 16-cell stage (n = 62, of which 60 showed pigment asymmetry). Note that the pigmentation of the epidermis is asymmetrical after inducing a new organizer displaced by 90° from its original site. The 40× digital magnification zoom of Axio Zoom V.16 Stereo Zoom Zeiss was used for images.