Fig. 1.

Nerve recording interface, experimental design, preprocessing methodological framework, and raw surface recordings before and after lidocaine administration. (A) Photograph of the bipolar cuff electrode recording activity from the surface of the cervical vagus nerve of mice. (B) Schematic diagram of the cytokine-injection experiments, with TNF injected first and IL-1β second or IL-1β injected first and TNF injected second. (C) Schematic diagram of the preprocessing data-analysis methodological framework with all the steps carried out to extract neural responses. (D) Trace of raw surface recordings during experiments where lidocaine was dropped distally on the cervical vagus nerve (the time of lidocaine administration is indicated by the blue arrow). (Upper) The complete recording. (Lower) A zoomed-in portion of the recording around the time of the lidocaine drop, with respiratory modulations colored red.