Fig. 1.

Water restriction selectively induced FXR expression in renal medullas of C57BL/6 mice. Male C57BL/6 mice were water-deprived for 24 h. (A) FXR mRNA expression was selectively induced in renal medulla. FXR mRNA levels were analyzed by qRT-PCR, *P < 0.05 vs. control (n = 4). (B) FXR protein expression was significantly increased in renal medulla as assessed by Western blot. Lamin A/C was used as protein loading control. (C) Statistical analysis of Western blot shown in B, **P < 0.01 vs. control (n = 4).