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. 2018 May 7;115(21):E4833–E4842. doi: 10.1073/pnas.1803564115

Fig. 2.

Fig. 2.

The psm3-A561E suppressor relieves noncleavable Rad21 toxicity. (A) Spot test results of six different strains containing the uncleavable rad21-13A or the hypercleavable rad21-13E mutation with or without cut1-L739S and hinge-destabilized psm3-A561E. (B) Streak test for colony formation of wild type or the psm3-A561E mutant carrying plasmid pRad21 wild type or the uncleavable pRad21-RERE mutant under the inducible promoter (+Thi off, −Thi on). psm3-A561E rescues the high toxicity of Rad21-RERE mutant protein overexpression.