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. 2018 May 7;115(21):E4833–E4842. doi: 10.1073/pnas.1803564115

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Copyright © 2018 the Author(s). Published by PNAS.

This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).

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Fig. 4. — The cut1 suppressors in Mis4/Scc2/NIPBL. (A) Surface potential of Mis4, based on the crystal structure of Scc2. Large positively charged (blue) areas that are likely to form a binding site for double-stranded DNA (schematically shown with magenta helices) are indicated by two green arrows. Inset shows an end view of DNA drawn to scale. (B) The architecture of the central part of Mis4/Scc2 and locations of the cut1 suppressors. Mutations are shown in green, and substitutions are shown with orange spheres. Heat-repeat motifs in which the mutations are identified are in magenta. (C) Boxed area in B, showing steric clashes caused by the P1035L mutation. (D) Spot test showing that all of the three cut1 suppressors in mis4 are UV damage sensitive. (E) Rad21 phosphorylation level (upper bands) detected using a polyclonal anti-Rad21 antibody. (F) Location of mis4-G1326E intragenic suppressors. (G) Locations of psm1 and psm3 head mutations that suppress mis4-G1326E or rad21-K1. rad21 suppressors are in blue, and mis4 suppressors are in magenta.