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. 2018 May 7;115(21):5468–5473. doi: 10.1073/pnas.1709211115

Fig. 4.

Fig. 4.

Impaired motility of TG2 phagosomes in WT primary mouse RPE cells. (A and B) Live-cell analysis of the tracks of phagosomes (85 per condition), from either WT or TG2 POSs, showed that TG2 phagosomes had a lower mean velocity (A) and shorter mean run length (B) than their WT counterparts. (C) Immunolabeling of RHO (green) and the endosomal marker RAB7A (red) in WT primary mouse RPE cells, fed either WT or TG2 POSs. (D) Quantification of the number of RHO-positive phagosomes colocalized with RAB7A showed that more phagosomes, derived from TG2 POSs relative to WT POSs, had RAB7A associated with them after a 1-h chase period. (E) Immunofluorescence of cytoplasmic dynein intermediate chain (DIC, green) and RHO (red) in WT primary mouse RPE cells fed POSs after a 1-h chase. White arrowheads in the merged panel indicate phagosomes associated with DIC immunolabeling, shown at higher magnification Below. (F) Quantification of the proportion of WT or TG2 POS phagosomes in WT primary mouse RPE cells that were associated with DIC immunolabel, after a 20-min pulse and a 1-h chase. (Scale bars in C and E: 20 μm and 5 μm, respectively.) Error bars in B, D, and F represent ±SEM. *P < 0.05; **P < 0.01; ****P < 0.0001.