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. 2018 Jun 1;9(42):26884–26899. doi: 10.18632/oncotarget.25529

Figure 3. The effect of TNFAIP8 on the expression of cell-cycle-related proteins.

Figure 3

(A) PC3 cells were transfected with empty vector or TNFAIP8-Myc plasmid for 30 h. The expression of the TNFAIP8-Myc protein in cell extracts was analyzed by immunoblotting with anti-Myc and anti-GAPDH antibodies (upper panel). PC3 cells were transfected with empty vector or TNFAIP8-Myc plasmid for 30 h, and total RNA was isolated. The effect of TNFAIP8 gene expression on the expression of different cell-cycle-related genes was analyzed using microarray (lower panel). Fold-change represents the relative gene expression changes in empty-vector- verses TNFAIP8-Myc-transfected PC3 cells. (B) PC3 cells were transfected with empty vector or TNFAIP8-Myc plasmid for 30 h. The effect of expression of the TNFAIP8-Myc plasmid on the regulation of cell cycle-related genes was analyzed by RT/qPCR as described in materials and methods section. (C) PC3 cells were transfected with empty vector or TNFAIP8-Myc plasmid for 30 h. The effect of TNFAIP8 protein on the expression of cell cycle-related proteins was analyzed by immunoblotting of the cell extracts with antibodies against p21, p27, and phosphorylated Histone- H3, CDC2, and Wee1, and other indicated antibodies. Data are expressed as the mean ± S.D. **p < 0.01, ***p < 0.001, according to the two-tailed Student's t-test.