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. 2018 Jun 1;9(42):26884–26899. doi: 10.18632/oncotarget.25529

Figure 5. TNFAIP8 interacts with ATG3, induces autophagy, and inhibits apoptosis.

Figure 5

(A) PC3 cell extracts (50 μg) from empty vector- or TNFAIP8-Myc-transfected cells were immunoblotted with anti-Myc, anti-ATG3, and anti-GAPDH antibodies (input panel). (B) Cell lysates (1 mg) from empty vector- or TNFAIP8-Myc-transfected PC3 cells were immunoprecipitated with control IgG or anti-ATG3 antibody and immunoblotted with anti-TNFAIP8-Myc and anti-ATG3 antibody. LC- light-chain IgG, HC- heavy-chain IgG. (C) PC3 cells were transfected with empty vector or TNFAIP8-Myc plasmid and treated with 3-methyladenine (4 mM) and TNFα (10 ng/ml) for 24 h as indicated. Cell extracts (50 μg) were immunoblotted with anti-Myc, anti-TNFAIP8, anti-LC3β I/II, anti-cleaved PARP, and anti-GAPDH antibodies.