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. 2018 Jun 1;9(42):26884–26899. doi: 10.18632/oncotarget.25529

Figure 6. TNFAIP8 increases the expression of NED biomarkers in prostate cancer PC3 cells.

Figure 6

(A) PC3 cells were transfected with empty vector or increasing amounts of TNFAIP8-Myc plasmid for 30 h. The effect of TNFAIP8 protein expression on NED biomarkers, such as chromogranin A, synaptophysin, NSE, and NCAM-1/CD56, as well as the autophagy marker LC3β I/II was analyzed by immunoblotting. (B) Effect of stable TNFAIP8 expression on NED markers in PC3 cells. Cell extracts from PC3 cells stably expressing TNFAIP8 were analyzed by immunoblotting with the indicated antibodies. (C) PC3 cells were transfected with empty vector or TNFAIP8-Myc plasmid and treated with 3-methyladenine for 24 h. The effect of TNFAIP8 expression, with and without 3-methyladenine, on the expression of NED and autophagy markers, was analyzed by immunoblotting.