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. Author manuscript; available in PMC: 2019 Jun 1.
Published in final edited form as: Proteomics. 2018 Mar 14;18(12):e1700246. doi: 10.1002/pmic.201700246

Figure 2. Analysis of the soluble HLA class I and class II complexes from plasma.

Figure 2

(A) Length distribution of sHLA-I peptides isolated from 4 donors (D1 to D4). The number of peptides between 7 and 20 amino acids is plotted in relation to their length. (B) NetMHCpan 3.0 binding prediction of 8 to 11-mers identified from plasma. Peptides were annotated as being predicted to bind if the rank received from NetMHCpan was below 2% for at least one of the alleles of the respective donor. (C, D) Length distribution of sHLA-II peptides isolated from plasma. sHLA-II DR peptides (C) or sHLA-II DQ peptides (D) were isolated from 2.5-3 mL of plasma from four donors (D1 to D4). The number of peptides between 6 and 30 amino acids is plotted in relation to their length.