Criteria	Number	Issue	Where to place information
Antibodies	For any antibody (including secondary antibodies) used, the Methods section should include the following:		Methods
	1a	The commercial (or other) source.	Methods
	1b	The species in which the antibody was raised, catalogue and batch/lot numbers.	Methods
	1c	The epitope against which it was raised.	Methods
	1d	The isotype (IgG, IgM, IgY, etc) and clone numbers (if applicable).	Methods
	1e	When available, https://scicrunch.org/resources.	Methods
	1f	The diluting buffer, the final antibody dilution and number of times solutions have been re‐used.	Methods
Controls	2	Positive and negative controls should be used, as much as possible.	Results
Images for review	3	Full uncropped images should be made available to reviewers and editors.	Results
Presentation	4	Separate immunoblots should NEVER be merged in figures.	Results
Normalisation	5	For Western blotting normalisation to the loading control should be done only if the bands for the target protein and the loading control are obtained from the same blot.	Results
Quantitation	6	Quantitation of band density can only be conducted on analysis within the linear range.	Results
Statistical comparisons	7	Statistical comparisons should only be carried out between bands on the same blot.	Results
Blinding	8	Full details of blinding for analysis of images should be provided.	Methods