Figure 3.

Species orthologues of FFA2 receptors display G protein selectivity. SCFAs promote phosphorylation and activation of ERK1/2 MAP kinases in HEK293 cells transfected to express either human or mouse FFA2. However, combinations of studies with the G protein inhibitors Pertussis toxin (G_i) and FR900359 (G_q/G₁₁) and the G_i‐biased FFA2 agonist AZ1729 demonstrate that while activation of the human orthologue transmits this signal largely via G_q/G₁₁, for the mouse orthologue the signal is transmitted largely via G_i. The indicated size of the noted G protein subtypes illustrates the relative contribution of each to SCFA‐mediated activation of ERK1/2 MAP kinases. See text and Bolognini et al. (2016b) for further details.