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. 2018 May 22;175(13):2689–2708. doi: 10.1111/bph.14333

Figure 2.

Figure 2

PZC, PZD and PZE reduce EMT and enhance podocyte marker in the TGFβ1‐ or AngII‐induced HK‐2 cells and podocytes. HK‐2 cells or podocytes were cultured in a serum‐fasted medium and treated with TGFβ1 or AngII (n = 6). (A) HK‐2 cells stimulated by TGFβ1 were stained with primary antibody against vimentin. The in situ expression of vimentin was analysed by Laser scanning confocal microscopes. Representative immunofluorescent staining images of TGFβ1‐induced vimentin expression in the different groups. (B) Protein expression and (C) quantitative analysis in the HK‐2 cells induced by TGFβ1. (D) Protein expression and (E) quantitative analysis in the HK‐2 cells induced by AngII. qRT‐PCR analysis of the mRNA expression of genes encoding collagen I, fibronectin, α‐SMA and E‐cadherin in the HK‐2 cells stimulated by TGFβ1 (F) or AngII (G). # P < 0.05 compared with control group (n = 6); * P < 0.05, compared with TGFβ1‐ or AngII‐ stimulated group (n = 6).