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. 2018 May 22;175(13):2689–2708. doi: 10.1111/bph.14333

Figure 4.

Figure 4

PZC, PZD and PZE inhibited the up‐regulation of Wnt1/β‐catenin signalling in the TGFβ1‐ or AngII‐stimulated HK‐2 cells and podocytes. (A) Protein expression and (B) quantitative analysis of Wnt1, active β‐catenin, β‐catenin and its target proteins Snail1, Twist, MMP7, PAI‐1 and FSP1 in the HK‐2 cells induced by TGFβ1. (C) Protein expression and (D) quantitative analysis of Wnt‐1/β‐catenin signalling in the HK‐2 cells induced by AngII. (E) HK‐2 cells stimulated by TGFβ1 were stained with primary antibody against β‐catenin. The in situ expression of β‐catenin was analysed. Representative immunofluorescent staining images of TGFβ1‐induced β‐catenin expression in the different groups. # P < 0.05 compared with control group (n = 6); * P < 0.05 compared with TGFβ1‐ or AngII‐ stimulated group (n = 6).