Skip to main content
. 2018 May 22;175(13):2689–2708. doi: 10.1111/bph.14333

Figure 7.

Figure 7

PZC, PZD and PZE block the interaction between TGFβ receptors and Smad in HK‐2 cells. (A) Cell lysates were immunoprecipitated with anti‐TGFBR1, followed by immunoblotting using antibodies against p‐Smad2, Smad2, p‐Smad3, Smad3 and TGFBR1. (B) Cell lysates were immunoprecipitated with anti‐Smad2, followed by immunoblotting using antibodies against TGFBR2, TGFBR1, p‐Smad2 and Smad2 (n = 6). (C) Cell lysates were immunoprecipitated with anti‐Smad3, followed by immunoblotting using antibodies against TGFBR2, TGFBR1, p‐Smad3 and Smad3. # P < 0.05 compared with control group (n = 6); * P < 0.05 compared with TGF β1‐stimulated group (n = 6). IP, immunoprecipitation; IB, immunoblotting.