Fig. 1.

IFNγ delays, but does not prevent, mortality despite higher viral load in infected CD46+ neonates.

A. Kaplan-Meier plot of CD46+ neonates on various knockout backgrounds. CD46+, CD46+/IFNγ-KO, and CD46+/RAG2-KO neonates were infected intracranially with measles virus (MV) (10^4 PFU/10 μl PBS) at 2 days of age. Mice were monitored for symptoms of illness and death for 35 days post-infection. Statistical analysis was applied by log rank test (p < 0.0001). Results from 4 to 6 separate litters were pooled (n = 30–50 mice per condition).

Whole brain lysates from neonatal (B) and adult (C) MV-infected mice were collected at 4 and 6 dpi. RNA levels of measles virus nucleocapsid (N) transcript were quantified using qRT-PCR. Bars represent the average of mice from three independent experiments (n = 9–14) and error bars represent SD. Statistical analysis was applied by two-way ANOVA (#p < 0.001, *p < 0.05, NS = not significant) with Bonferroni post hoc test.