Fig. 8.

Neonatal mice induce greater expression of Type I interferons during MV infection in comparison to adults.

Brains of uninfected and MV-infected CD46+ mice were analyzed for the mRNA expression of Type I interferons at 3 dpi and 7 dpi. CD46+, CD46+/IFNγ-KO, and CD46+/RAG2-KO neonates (left column; A, C, E) and CD46+ neonates and adults (right column; B, D, F) were compared. qRT-PCR analysis was performed for IFNα4 (A, B), IFNβ (C, D) and MDA5 (E, F). Relative gene expression is shown as the fold-change normalized to the CD46+ uninfected controls (n = 4–5 mice/condition). Each bar represents the mean fold-change and SEM. Statistical differences were determined by three-way ANOVA (*p < 0.05, ^#p < 0.001) with Bonferroni post hoc test.